A 200 mM stock of Methyl-β-cyclodextrin is not recommended due to its maximal solubility of 50 mg/mL. Since the molecular mass of MβCD is 1310, the highest recommended concentration is 38 mM.
C4555
Methyl-β-cyclodextrin
powder, BioReagent, suitable for cell culture
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
1 g | Estimated to ship onMay 26, 2026fromMILWAUKEE | $101.00 |
5 g | Estimated to ship onMay 26, 2026fromMILWAUKEE | $359.00 |
10 g | Estimated to ship onMay 26, 2026fromMILWAUKEE | $582.00 |
About This Item
Form:
powder
$101.00
Estimated to ship onMay 26, 2026Details
Subscriptions are currently unavailable.
Ships Every 4 weeks
biological source
synthetic (organic)
Quality Segment
sterility
non-sterile
product line
BioReagent
form
powder
mol wt
~1320 g/mol
extent of labeling
1.5-2.1 methyl per mol glucose
technique(s)
cell culture | mammalian: suitable
mp
180-182 °C (lit.)
solubility
H2O: 50 mg/mL
storage temp.
room temp
SMILES string
O1C2OC(C(OC3OC(C(OC4OC(C(OC5OC(C(OC6OC(C(OC7OC(C(OC8OC(C1C(C8OC)O)COC)C(C7OC)O)COC)C(C6OC)O)COC)C(C5OC)O)COC)C(C4OC)O)COC)C(C3OC)O)COC)C(C2OC)O)COC
InChI
1S/C56H98O35/c1-64-15-22-36-29(57)43(71-8)50(78-22)86-37-23(16-65-2)80-52(45(73-10)30(37)58)88-39-25(18-67-4)82-54(47(75-12)32(39)60)90-41-27(20-69-6)84-56(49(77-14)34(41)62)91-42-28(21-70-7)83-55(48(76-13)35(42)63)89-40-26(19-68-5)81-53(46(74-11)33(40)61)87-38-24(17-66-3)79-51(85-36)44(72-9)31(38)59/h22-63H,15-21H2,1-14H3
InChI key
QGKBSGBYSPTPKJ-UHFFFAOYSA-N
General description
Methyl-β-cyclodextrin is a heptasaccharide, soluble in water, and has more affinity to cholesterol due to the presence of hydrophobic core. Cyclodextrins are cyclic oligosaccharides consisting of 6, 7, or 8 glucopyranose units, usually referred to as α-, β-, or γ-cyclodextrins, respectively. These compounds have rigid doughnut-shaped structures making them natural complexing agents. The unique structures of these compounds owe their stability to intramolecular hydrogen bonding between the C2- and C3-hydroxyl groups of neighboring glucopyranose units. The molecule takes on the shape of a torus with the C2- and C3-hydroxyls located around the larger opening and the more reactive C6-hydroxyl aligned around the smaller opening. The arrangement of C6-hydroxyls opposite the hydrogen bonded C2- and C3-hydroxyls forces the oxygen bonds into close proximity within the cavity, leading to an electron rich, hydrophobic interior. The size of this hydrophobic cavity is a function of the number of glucopyranose units forming the cyclodextrin.
The solubility of natural cyclodextrins is very poor. In the late 1960′s, it was discovered that chemical substitutions at the 2, 3, and 6 hydroxyl sites would greatly increase solubility. Most chemically modified cyclodextrins are able to achieve a 50% (w/v) concentration in water.
The solubility of natural cyclodextrins is very poor. In the late 1960′s, it was discovered that chemical substitutions at the 2, 3, and 6 hydroxyl sites would greatly increase solubility. Most chemically modified cyclodextrins are able to achieve a 50% (w/v) concentration in water.
Application
Methyl-β-cyclodextrin has been used:
- to study the effect of cholesterol depletion, from sperm membrane on sperm′s ability to undergo acrosome reaction.
- to determine the effect of caveolin overexpression and its loss on pro-survival and pro-growth signaling
- in conventional in vitro fertilization
Biochem/physiol Actions
Methyl-β-cyclodextrin (MβCD) is commonly applicable as an inhibitor and modifies cholesterol domains on the cellular surface. It causes depletion of cholesterol on the membrane. It is also known to prevent the action of cholera toxin, nitric oxide (NO) synthase and glucose transporter through internalization. This is observed in specific to lipid rafts and caveolae endocytosis. MβCD induces eflux as well as influx of cholesterol. It can affect cell viability as the genes regulating cholesterol levels are upregulated, due to decreased cholesterol level by the action of MβCD. It can be used to increase the solubility of non-polar substances such as fatty acids, lipids, vitamins and cholesterol for use in cell culture applications. Cavity size is the major determinant as to which cyclodextrin is used in complexation. The cavity diameter of β-cyclodextrins or β-glucopyranose unit compounds is well-suited for use with molecules the size of hormones, vitamins and many compounds frequently used in tissue and cell culture applications. For this reason, β-cyclodextrin is most commonly used as a complexing agent.
Preparation Note
Solutions may be obtained by stirring 30 min at room temperature. Alternatively, sonication with cooling may be employed. Solutions may be stored for several months at 4°C. Solid should be stored tightly sealed at room temperature.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 300 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility - |
| form powder | form powder | form powder | form powder |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. - |
| mp 180-182 °C (lit.) | mp 180-182 °C (lit.) | mp 180-182 °C (lit.) | mp 180-182 °C (lit.) |
| extent of labeling 1.5-2.1 methyl per mol glucose | extent of labeling 1.6-2.0 mol CH3 per unit anhydroglucose | extent of labeling 1.6-1.9 molar substitution | extent of labeling - |
Still not finding the right product?
Explore all of our products under Methyl-β-cyclodextrin
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
368.6 °F
flash_point_c
187 °C
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
How to make a 200 mM stock of methy Beta cyclodexterin ? Solublilty is 50mg/liter
1 answer-
Helpful?
-
-
What is the molecular weight of Methyl-β-cyclodextrin, Product No. C4555?
1 answer-
The approximate molecular weight is 1320 g/mol. The molecular weight can vary from lot to lot due to variation in the methyl content of the product. The molecular weight of this product is dependent on the average number of methyl groups per glucose residue (reported on the lot specific Certificate of Analysis). View the specification sheet above.
Helpful?
-
-
How does Methyl-β-cyclodextrin, Product C4555, work to solubilize products that have minimal solubility in aqueous solutions for use in cell culture?
1 answer-
The cavity diameter of β -cyclodextrins compounds is well-suited for use with molecules the size of hormones, vitamins and many compounds frequently used in tissue and cell culture applications. For this reason, β-cyclodextrin is most commonly used as a complexing agent. Hydrophobic molecules are incorporated into the cavity of cyclodextrins by displacing water. This reaction is favored by the repulsion of the molecule by water. This effectively encapsulates the molecule of interest within the cyclodextrin, rendering the molecule water soluble. When the water soluble complex is diluted in a much larger volume of aqueous solvent, the process is reversed, thereby releasing the molecule of interest into the solution.
Helpful?
-
-
What is Product C4555, Methyl-β-cyclodextrin?
1 answer-
Methyl-β-cyclodextrin is used as a "carrier" molecule to facilitate the dissolution of compounds with low solubility in water.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How should a solution of Methyl-β-cyclodextrin, Product C4555, be sterilized?
1 answer-
Solutions can be sterile filtered using a 0.2 μm membrane. We do not recommend autoclaving the solution. The product as supplied is not sterile.
Helpful?
-
-
How can I make a solution of Methyl-β-cyclodextrin, Product C4555?
1 answer-
This product is tested and found soluble at 50 mg/ml in 1 M NaOH yielding a clear, colorless solution. This product is also soluble in water (150 mg/ml), yielding a clear, colorless solution. Aqueous solutions may be obtained by stirring 30 minutes at room temperature. Alternatively, sonication with cooling may be employed.
Helpful?
-
-
What concentration of Methyl-β-cyclodextrin, Product C4555, do I use to remove cholesterol from cell membranes?
1 answer-
This product has been used to reduce the cholesterol content of cell membranes at 10 - 50 mM.References:Eur J Cell Biol. 1999 Jul, 78(7): 473-84.Lipids. 1996 Aug, 31(8): 817-28.
Helpful?
-