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C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Synonym(s):

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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Pack SizeSKUAvailabilityPrice
1500 units

Estimated to ship onAugust 11, 2026fromMILWAUKEE

$350.00
3000 units

Estimated to ship onAugust 11, 2026fromMILWAUKEE

$632.00
7500 units

Available to ship TODAYfromMILWAUKEE

$1,260.00
$1,071.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥100 units/mg protein

$350.00


Estimated to ship onAugust 11, 2026Details


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form

lyophilized powder

Quality Segment

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2]

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[3]

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

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G2878C1129T9034
specific activity

≥100 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

lyophilized powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

shipped in

wet ice

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥50% biuret

composition

-

composition

-

composition

-


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Global Trade Item Number

SKUGTIN
C4018-7.5KU04061832755328
C4018-3KU04061832755311
C4018-1.5KU04061832755304

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