C2542

Monoclonal Anti-N-Cadherin antibody produced in mouse

clone GC-4, ascites fluid

• Synonym(s): Anti-A-CAM, Anti-A-Cell Adhesion Molecule
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164511
• Conjugate: unconjugated
• Clone: GC-4, monoclonal
• Application: ARR, EM, IHC (f), WB
• Citations: 16

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: GC-4, monoclonal
• contains: 15 mM sodium azide
• species reactivity: chicken, mouse, human, rabbit, rat
• technique(s): electron microscopy: suitable, immunohistochemistry (frozen sections): 1:100 using chicken cardiac muscle, microarray: suitable, western blot: 1:100 using chicken or rat cardiac muscle
• UniProt accession no.: P19022
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDH2(1000) ; mouse ... Cdh2(12558) ; rat ... Cdh2(83501)

Description

General description

N-cadherin is a calcium dependent cell adhesion molecule and is present mainly in cancer cells. Monoclonal anti-N-cadherin antibody reacts with the N-terminal portion of the extracellular domain of adipocyte adhesion molecule (ACAM).

Immunogen

Affinity purified chicken heart A-CAM.

Application

Monoclonal anti-N-cadherin antibody can be used in immunofluorescent microscopy, electron microscopy and immunoblotting to study the receptor localization of intercellular adherens. It may also be used to study the mechanism of adhesion and interaction in tissues.

Monoclonal anti-N-cadherin antibody can be used in immunohistochemistry (1: 100) and as primary antibody in western blotting (1:1000) .

Biochem/physiol Actions

Monoclonal anti-N-cadherin antibody reacts specifically with A-CAM molecule in human, mouse, rabbit, chicken and rat.

N-cadherin regulates the Src kinase pathway and also promotes cell motility and invasion. It inhibits adherens junction formation and disrupts existing junctions of cultured lens cells.

Physical form

Monoclonal Anti-N-Cadherin antibody produced in mouse is supplied as ascites fluid containing 15mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable