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Cholesterol Esterase from Pseudomonas sp.

Name: Cholesterol Esterase from <I>Pseudomonas</I> sp.
Brand: SIGMA
Price: 346 USD
Availability: InStock

lyophilized powder, ≥200,000 units/g protein

Synonym(s):

Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase

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Pack Size	SKU	Availability	Price
25 units		Available to ship TODAYfromMILWAUKEE	$346.00
100 units		Estimated to ship onMay 28, 2026fromMILWAUKEE	$985.00

About This Item

CAS Number:

9026-00-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-808-6

MDL number:

MFCD00071052

EC Number:

3.1.1.13 (BRENDA, IUBMB)

Specific activity:

≥200,000 units/g protein

$346.00

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥200,000 units/g protein

mol wt

~300 kDa

composition

protein, ≥40% biuret

storage temp.

−20°C

Description

General description

Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾⁵M (Linoleate), 6.6 x 10‾⁵M (Oleate)
3.7 x 10‾⁵M (Linolenate), 1.5 x 10‾⁴M (Palmitate)
1.2 x 10‾⁴M (Myristate), 2.3 x 10‾⁵M (Stearate)
Inhibitors: Hg^++,Ag⁺, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)

Application

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.[1]

Other Notes

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

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This Item	C9281	C3766	228180
Sigma-Aldrich C1403 Cholesterol Esterase from Pseudomonas sp.	Sigma-Aldrich C9281 Cholesterol Esterase from Pseudomonas fluorescens	Sigma-Aldrich C3766 Cholesterol Esterase from bovine pancreas	Sigma-Aldrich 228180 Cholesterol Esterase, Microbial
specific activity ≥200,000 units/g protein	specific activity ≥10,000 units/g protein	specific activity ≥200 units/g protein	specific activity ≥100 units/mg protein, ≥70 units/mg dry wt.
form lyophilized powder	form lyophilized powder	form lyophilized powder	form powder
mol wt ~300 kDa	mol wt ~129 kDa	mol wt 84 kDa by SDS-PAGE	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
composition protein, ≥40% biuret	composition Protein, ~20%	composition protein, ≥20%	composition -