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Carboxypeptidase A−Agarose

Name: Carboxypeptidase A−Agarose
Brand: SIGMA
Price: 250 USD
Availability: InStock

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Synonym(s):

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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Pack Size	SKU	Availability	Price
10 units		Check Cart for Availability	$250.00
25 units		Check Cart for Availability	$497.00

About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.17.1 (BRENDA, IUBMB)

MDL number:

MFCD00130718

Specific activity:

≥6 units/mL packed gel, 25 °C

Biological source:

enzyme from bovine pancreas

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Properties

biological source

enzyme from bovine pancreas

Quality Segment

200

form

ammonium sulfate suspension

specific activity

≥6 units/mL packed gel, 25 °C

mol wt

~35,250

matrix

beaded agarose

storage temp.

2-8°C

Description

General description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.[1]

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

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This Item	C9268	T1763	C9584
Sigma-Aldrich C1261 Carboxypeptidase A−Agarose	Sigma-Aldrich C9268 Carboxypeptidase A from bovine pancreas	Sigma-Aldrich T1763 Trypsin Agarose	Sigma-Aldrich C9584 Carboxypeptidase B from porcine pancreas
biological source enzyme from bovine pancreas	biological source -	biological source bovine pancreas (trypsin)	biological source Porcine pancreas
specific activity ≥6 units/mL packed gel, 25 °C	specific activity ≥50 units/mg protein	specific activity -	specific activity ≥125 units/mg protein
form ammonium sulfate suspension	form ready-to-use solution	form buffered aqueous suspension	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
mol wt ~35,250	mol wt ~35 kDa	mol wt -	mol wt 34,000 Da± 600
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200