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Cholesterol Oxidase microbial

Name: Cholesterol Oxidase microbial
Brand: SIGMA
Price: 277 USD
Availability: InStock

recombinant, lyophilized powder, ≥10 units/mg protein

Synonym(s):

Cholesterol: oxygen oxidoreductase

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Pack Size	SKU	Availability	Price
100 units		Check Cart for Availability	$277.00

About This Item

CAS Number:

9028-76-6

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-842-1

MDL number:

MFCD00130783

EC Number:

1.1.3.6 (BRENDA, IUBMB)

Specific activity:

≥10 units/mg protein

$277.00

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Properties

Quality Segment

200

form

lyophilized powder

specific activity

≥10 units/mg protein

mol wt

55 kDa

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble

storage temp.

−20°C

Description

Application

Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H₂O₂. Optimum pH of the enzyme is 7.0. Hg²⁺, Ag⁺, ionic detergents inhibit the enzyme activity.

Preparation Note

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

Other Notes

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system.

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This Item	C8868	C5421	C8649
Sigma-Aldrich C1235 Cholesterol Oxidase microbial	Sigma-Aldrich C8868 Cholesterol Oxidase from microorganisms	Sigma-Aldrich C5421 Cholesterol Oxidase from microorganisms	Sigma-Aldrich C8649 Cholesterol Oxidase from Streptomyces sp.
specific activity ≥10 units/mg protein	specific activity ≥50 units/mg protein	specific activity ≥30 units/mg protein (biuret)	specific activity ≥20 units/mg protein
form lyophilized powder	form lyophilized powder	form aqueous solution	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −70°C	storage temp. −20°C
solubility 50 mM potassium phosphate buffer, pH 7.0: soluble	solubility -	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
mol wt 55 kDa	mol wt 64 kDa	mol wt 62 kDa	mol wt ~34 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200