C0897
Citrate Lyase from Klebsiella pneumoniae
lyophilized powder, ≥0.20 unit/mg solid
Synonym(s):
Citrate oxaloacetate lyase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 units | Check Cart for Availability | $352.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-740-7
MDL number:
Specific activity:
≥0.20 unit/mg solid
$352.00
Check Cart for Availability
form
lyophilized powder
Quality Level
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
Application
Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).
Biochem/physiol Actions
Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).[1]
Physical form
Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4
Other Notes
One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥0.20 unit/mg solid | specific activity ≥0.25 U/mg (At 25 °C with citrate as the substrate.) | specific activity ≥0.1 unit/mg solid | specific activity 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) |
| form lyophilized powder | form lyophilized | form powder | form lyophilized powder |
| mol wt >5 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0897-100UN | 04061835512256 |