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C0794

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Synonym(s):

Chitosan N-acetylglucosaminohydrolase

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10 units
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$508.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥15 units/mg protein (E1%)
Concentration:
≥0.5 mg/mL

$508.00


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form

buffered aqueous glycerol solution

Quality Segment

specific activity

≥15 units/mg protein (E1%)

concentration

≥0.5 mg/mL

solubility

0.1 M acetate buffer, pH 5.5: soluble

storage temp.

−20°C

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.[1]

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5[1]

Other Notes

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

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This Item
C9830C8649A9228
specific activity

≥15 units/mg protein (E1%)

specific activity

>50 units/mg protein (Bradford)

specific activity

≥20 units/mg protein

specific activity

≥40,000 units/g solid

concentration

≥0.5 mg/mL

concentration

-

concentration

-

concentration

-

form

buffered aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized solid

solubility

0.1 M acetate buffer, pH 5.5: soluble

solubility

H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

solubility

0.03 M sodium citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
C0794-10UN04061833452202

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