The Casein used to prepare this product is a mixture of Alpha-s1, Alpha-s2, Beta, and Kappa Casein. Combined, these forms of casein have a molecular weight range of 19 - 25.2 kDa. The Fluorescein isothiocyanate I isomer has a molecular weight of 389.38. The ratio of casein to FITC is approximately 11:1. A confirmed molecular weight has not been established.
C0528
Casein fluorescein isothiocyanate from bovine milk
Type III, essentially salt-free, lyophilized powder
Synonym(s):
FITC-casein
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Available to ship TODAYfromMILWAUKEE | $239.00 | |
| 25 mg | Available to ship TODAYfromMILWAUKEE | $395.00 | |
| 100 mg | Available to ship TODAYfromMILWAUKEE | $1,180.00 |
About This Item
type
Type III
Quality Segment
form
essentially salt-free, lyophilized powder
extent of labeling
≥50 μg FITC per mg solid
solubility
water: 5 mg/mL, clear to hazy
storage temp.
2-8°C
Application
highly sensitive protease substrate
Casein fluorescein isothiocyanate from bovine milk has been used:
- in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
- to make a reference line in immunochromatographic devices
- in running gel for the documentation of proteinases
Biochem/physiol Actions
Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.
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This Item | |||
|---|---|---|---|
| solubility water: 5 mg/mL, clear to hazy | solubility water: 5 mg/mL, clear to hazy, yellow to orange | solubility - | solubility H2O: 25 mg/mL, clear to slightly hazy, yellow-orange |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| type Type III | type Type II | type - | type - |
| extent of labeling ≥50 μg FITC per mg solid | extent of labeling 20-50 μg FITC per mg solid | extent of labeling - | extent of labeling 0.003-0.020 mol FITC per mol glucose |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0528-10MG | 04061832908199 |
| C0528-25MG | 04061832908205 |
| C0528-100MG | 04061833450758 |
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What's the mwt of fitc-casein in kda?
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