Skip to Content
MilliporeSigma

Skip To

B8781

Restriction Enzyme Buffer SB

10 ×

Synonym(s):

Restriction Endonuclease Buffer

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
5 x 1 vial
Check Cart for Availability
$64.70

About This Item

NACRES:
NA.53
UNSPSC Code:
12352204

$64.70


Check Cart for Availability

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


grade

Molecular Biology

Quality Segment

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

form

buffered aqueous solution

concentration

10 ×

storage temp.

−20°C

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Application

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
S6639S2015B6429
grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

concentration

10 ×

concentration

-

concentration

-

concentration

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

solution

form

solution

form

solution

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

description

Saline-Sodium Citrate buffer, made with ultrapure water

description

-

description

-


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library


Articles

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.






Global Trade Item Number

SKUGTIN
B8781-5X1VL04061833294710
B8781-1VL04061837769887

Questions

Reviews

No rating value

Active Filters