B8395

Anti-Bovine IgG (whole molecule) antibody produced in rabbit

whole antiserum

• MDL number: MFCD00162167
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:150,000; quantitative precipitin assay: 4.0 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

Specificity

The antiserum is determined to be immunospecific for bovine IgG.

Application

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used as a standard to determine protein content by Bradford method using Bio-Rad protein assay dye reagent. It has also been used in several assays to develop a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk.

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used for detecting IgG antibodies in multichannel monolithic quartz crystal microbalance (MQCM).

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulins G (IgGi, IgGa) of bovine milk is capable of protecting the gut mucosa against microorganisms.

Physical form

Rabbit Anti-Bovine IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative

Preparation Note

treated to remove lipoproteins

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg