B7554

Anti-c-Myc−Biotin antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• Synonym(s): Anti-c-Myc, Monoclonal Anti-c-Myc−Biotin antibody produced in mouse
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: 9E10, monoclonal
• Application: WB CL
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: biotin conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 0.8 mg/mL
• technique(s): western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein
• UniProt accession no.: P01106
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYC(4609)

Description

General description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

The antibody is suitable for immunoblotting.

Biochem/physiol Actions

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable