A9934
Aminopeptidase I from Streptomyces griseus
lyophilized powder, ≥200 units/mg protein
Synonym(s):
Leucine Aminopeptidase IV
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0.1 MG
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$287.30
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-874-6
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein
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form
lyophilized powder
Quality Level
specific activity
≥200 units/mg protein
mol wt
21 kDa by gel filtration, 33 kDa by SDS-PAGE
composition
Protein, 40-60% Lowry
storage temp.
−20°C
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| specific activity ≥200 units/mg protein | specific activity ≥3.5 units/mg solid | specific activity ≥5.0 units/mg protein | specific activity 0.5 units/mg protein |
| form lyophilized powder | form powder | form lyophilized powder | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 21 kDa by gel filtration, 33 kDa by SDS-PAGE | mol wt - | mol wt 24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE | mol wt 37 kDa by SDS-PAGE |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, 40-60% Lowry | composition - | composition - | composition - |
General description
Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues.[1]
Application
Biochem/physiol Actions
Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn2+ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca2+, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.
Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.
Packaging
Package size based on protein content.
Physical form
Contains calcium acetate
Preparation Note
Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.
Other Notes
Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.)
One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0, 25 °C and 3.0 mM substrate concentration.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Jason L Parsons et al.
The FEBS journal, 272(22), 5753-5763 (2005-11-11)
Ionizing radiation, oxidative stress and endogenous DNA-damage processing can result in a variety of single-strand breaks with modified 5' and/or 3' ends. These are thought to be one of the most persistent forms of DNA damage and may threaten cell
Bonnie K Baxter et al.
The Journal of biological chemistry, 280(47), 39067-39076 (2005-09-28)
The cytoplasm to vacuole (Cvt) trafficking pathway in S. cerevisiae is a constitutive biosynthetic pathway required for the transport of two vacuolar enzymes, aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p), to the vacuole. Ape1p and Ams1p bind to their receptor, Atg19p
[Cytoplasm to vacuole targeting pathway in yeast].
Takahiro Shintani
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 51(10 Suppl), 1480-1483 (2006-08-23)
Wakana Adachi et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 63(Pt 3), 200-203 (2007-03-03)
The vacuole hydrolase aminopeptidase 1 (Ape1) is a cargo protein transported to the vacuole by the cytosol-to-vacuole targeting (Cvt) pathway during conditions of growth and by autophagy during conditions of starvation. After transport to the vacuole, Ape1 is processed into
Jem A Efe et al.
Journal of cell science, 118(Pt 20), 4751-4764 (2005-10-13)
Although the small Arf-like GTPases Arl1-3 are highly conserved eukaryotic proteins, they remain relatively poorly characterized. The yeast and mammalian Arl1 proteins bind to the Golgi complex, where they recruit specific structural proteins such as Golgins. Yeast Arl1p directly interacts
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9934-.1MG | 04061832699615 |
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