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A2765

Azocasein

protease substrate, chromogenic, powder

Synonym(s):

Sulfanilamide-azocasein

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Pack SizeSKUAvailabilityPrice
1 g

Available to ship TODAYfromMILWAUKEE

$69.80
5 g

Available to ship TODAYfromMILWAUKEE

$138.00
10 g

Available to ship TODAYfromMILWAUKEE

$237.00
25 g

Available to ship TODAYfromMILWAUKEE

$469.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:

$69.80


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Product Name

Azocasein, protease substrate

biological source

bovine

Quality Segment

form

powder

technique(s)

ligand binding assay: suitable

solubility

water: 5 mg/mL, clear, orange to very deep orange

ε (extinction coefficient)

≥25 at 440 nm in 0.1 M NaOH at 1%

storage temp.

2-8°C

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
Azocasein has been used as a substrate for determination of protease activity.

Other Notes


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This Item
A1888C3777A4737
technique(s)

ligand binding assay: suitable

technique(s)

-

technique(s)

-

technique(s)

ligand binding assay: suitable

solubility

water: 5 mg/mL, clear, orange to very deep orange

solubility

water: 10 mg/mL, clear to slightly hazy

solubility

water: 5 mg/mL, clear to hazy, yellow to orange

solubility

H2O: soluble 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

biological source

bovine

biological source

-

biological source

-

biological source

bovine


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Product Information Sheet






Global Trade Item Number

SKUGTIN
A2765-1G04061835504466
A2765-10G04061835553174
A2765-5G04061835553198
A2765-25G04061835553181

Questions

  1. A2765 Sigma-Aldrich アゾカゼイン この製品に含まれるアゾ色素を標品として使用したいのですが、どの化合物になるでしょうか?

    1 answer
    1. This product is prepared by reaction of the diazotized form of Sulfanilamide with casein. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/768/303/a2765pis.pdf

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