If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
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For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
A0512
Amylose from potato
used as amylase substrate
Synonym(s):
α-Amylose
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 250 mg | Available to ship TODAYfromMILWAUKEE | $62.20 | |
| 1 g | Available to ship TODAYfromMILWAUKEE | $108.00 | |
| 5 g | Available to ship TODAYfromMILWAUKEE | $515.00 | |
| 25 g | Available to ship TODAYfromMILWAUKEE | $2,570.00 |
About This Item
$62.20
Available to ship TODAYDetails
biological source
potato
Quality Segment
form
powder
impurities
amylopectin, essentially free, ≤10% ethanol, ≤2% butanol
color
white to off-white
solubility
0.05 M NaOH: 1 mg/mL, clear to slightly hazy, colorless
storage temp.
room temp
SMILES string
O1[C@@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)O)O)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O)CO
InChI
1S/C18H32O16/c19-1-4-7(22)8(23)12(27)17(31-4)34-15-6(3-21)32-18(13(28)10(15)25)33-14-5(2-20)30-16(29)11(26)9(14)24/h4-29H,1-3H2/t4-,5-,6-,7-,8+,9-,10-,11-,12-,13-,14-,15-,16+,17-,18-/m1/s1
InChI key
FYGDTMLNYKFZSV-PXXRMHSHSA-N
General description
Application
Biochem/physiol Actions
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| biological source potato | biological source - | biological source - | biological source sweet potato |
| solubility 0.05 M NaOH: 1 mg/mL, clear to slightly hazy, colorless | solubility - | solubility - | solubility - |
| Quality Level 300 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. room temp | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| impurities amylopectin, essentially free, ≤2% butanol, ≤10% ethanol | impurities - | impurities - | impurities - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A0512-250MG | 04061833334027 |
| A0512-1G | 04061835365944 |
| A0512-5G | 04061833334041 |
| A0512-25G | 04061833334034 |
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the purity of amylose of Product A0512?
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This product is tested for suitability as an amylase substrate. The purity is not determined. Note that this material may contain up to 10% ethanol and 2% butanol. This information is reported on a lot to lot basis. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/a0512#product-documentationHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What do the "Types" of Amylose from Potato, Product A0512, mean?
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The "Type" of the product has no significant meaning outside Sigma-Aldrich. The differences between products were designated by types before Sigma had established product numbers for specific products. The types were differentiated on several bases, including manufacturing method, source, and purity.
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What is the molecular weight of Amylose from Potato, Product A0512?
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Sigma-Aldrich has not determined the molecular weight of the product. One reported range is from 40,000 to 340,000 Daltons, with chains containing 250 to 2000 anhydroglucose units. Source: WHO Food Additives Series No. 5.
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What is the method of preparation of Amylose from Potato, Product A0512?
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The method of preparation is given in Street and Close, Clin. Chim. Acta, 1, 256-268 (1956). Potato starch is dissolved in boiling 2% (w/v) NaCl solution, filtered while hot, then cooled. Thymol is added, and the mixture is stirred for 48 hr. Amylopectin is decanted away in the supernatant. The precipitate is washed six times with concentrated thymol solution, four times with absolute ethanol, and dried.
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What is the blue value of Amylose from Potato, Product A0512? How is it determined?
1 answer-
Prepare iodine solution and Solution A: 1. Iodine solution: 0.2% iodine in 2% potassium iodide.2. Solution A: To 100 mg of sample in 100 mL flask, add 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Warm in boiling water bath until sample dissolves. After cooling, dilute to 100 mL with water.Transfer 2.5 mL of solution A to 250 mL flask. To this flask, add 50 mL water, then 2 drops of 6 N hydrochloric acid, followed by 2.5 mL of Iodine solution. Dilute to 250 mL with water. Read absorbance at 680 nm against a blank.The final solution to be read will be at a sample concentration of 10 μg per mL, and 1% of the iodine solution.This procedure is based on McCready, R. M., and Hassid, W. Z., The Separation and Quantitative Estimation of Amylose and Amyolpectin in Potato Starch. J. Am. Chem. Soc., Vol. 65, pp. 1154-1157 (1943).
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What can I use to solubilize Amylose from Potato, Product A0512?
1 answer-
This product is soluble in 50 mM NaOH at a concentration of 1 mg/mL. The product can also be dissolved as follows (final concentration = 1 mg/ml): Dissolve 100 mg in 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Heat in boiling water bath until sample dissolves, cool to ambient temperature, then dilute to 100 mL with water.
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