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63590

Sigma-Aldrich

May-Grünwald solution

for the staining of blood constituents

Synonym(s):

Eosin – methylene blue solution, Eosin methylene blue according to May-Grünwald

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About This Item

MDL number:
MFCD00005038
UNSPSC Code:
12171500
PubChem Substance ID:
329759877
NACRES:
NA.25

grade

for the staining of blood constituents

Quality Level

100

technique(s)

microbe id | staining: suitable

impurities

methanol

density

0.80 g/mL at 20 °C

suitability

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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Application

May-Grünwald solution (Eosin – methylene blue solution) is used with Giemsa stain for histological staining of blood cells.

Other Notes

Stain for the panoptic staining according to Pappenheim

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

51.8 °F - closed cup

flash_point_c

11 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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V Hofman et al.
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Elsa Beraki et al.
CytoJournal, 9, 8-8 (2012-05-11)
Protocols for immunocytochemical staining (ICC) and in situ hybridization (ISH) of air-dried Diff-Quick or May-Grünwald Giemsa (MGG)-stained smears have been difficult to establish. An increasing need to be able to use prestained slides for ICC and ISH in specific cases
Michał Zimecki et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 61(11), 1247-1255 (2012-07-20)
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Involvement of intrinsic mitochondrial pathway in neosergeolide-induced apoptosis of human HL-60 leukemia cells: The role of mitochondrial permeability transition pore and DNA damage.
Cavalcanti BC, da Costa PM, et al.
L' Eurobiologiste, 50, 980-993 (2012)
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