WBLUC
Immobilon® Classico Western HRP substrate
Western blotting, dot/slot blotting
Synonym(s):
HRP substrate, chemiluminescent detection substrate, detection substrate
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
Estimated to ship TODAY | $128.00 | ||
Estimated to ship TODAY | $277.00 |
About This Item
Product Name
Immobilon Classico Western HRP substrate, Western blotting, dot/slot blotting
conjugate
peroxidase conjugate
Quality Level
manufacturer/tradename
Luminata
technique(s)
western blot: suitable
membrane area
1000 cm2 , membrane coverage
detection method
chemiluminescent
shipped in
ambient
General description
Application
Packaging
Preparation Note
Analysis Note
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| membrane area 1000 cm2 , membrane coverage | membrane area 1000 cm2 , membrane coverage | membrane area 1000 cm2 , membrane coverage | membrane area 5000 cm2 , membrane coverage |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate |
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Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Articles
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Related Content
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| WBLUC0100 | 04053252288630 |
| WBLUC0500 | 04053252288647 |