ST1673

Anti-AFP Mouse mAb (1G7)

liquid, clone 1G7, Calbiochem^®

• Synonym(s): Anti-α-Fetoprotein
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 1G7, monoclonal
• Species reactivity: human
• Application: —
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1G7, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA (see coments); Immunoblotting (1-5 µg/mL); Immunocytochemistry (10 µg/mL))
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AFP(174)

Description

General description

Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.

Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.

Recognizes the ~70-75 kDa AFP protein in HepG2 cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein

Application

ELISA (see coments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

Physical form

In PBS, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
HepG2 cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable