MAC138

Anti-Inntag 5 (IT5) Antibody, clone R19/8-11/18

clone R19/8-11/18, from mouse

• Synonym(s): Pollen allergen Phl p 2, Phl p2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: R19/8-11/18, monoclonal
• Application: IF, IP, WB
• Citations: -

Properties

• Quality Level: 100
• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: R19/8-11/18, monoclonal
• species reactivity: Phleum pratense
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25 μL
• technique(s): immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: P43214
• shipped in: ambient
• target post-translational modification: unmodified

Description

General description

~40.2 kDa observed; 13.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Pollen allergen Phl p 2 (UniProt: P43214; also known as Allergen Phl p II, Phl p 2) is encoded by the PHLPII gene in Phleum pratense (Common timothy). Phl p2 binds to IgE and causes allergic reactions in human. Inntag 5 IT5 is a small protein domain of Phelum pratense that display strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Although the Inntag IT5 has a similar length to that of the Flag tag, it does not affect cell morphology and produces only very mild effect in the mean critical size at the G1/S transition compared to Flag tag. The structure of Inntag IT5 is based on a packed beta-sheet confirmation.

Immunogen

His-tagged recombinant fragment corresponding to 96 amino acids of Pollen allergen Phl p 2 chain.

Application

Anti-Inntag 5 (IT5), clone R19/8-11/18 is a mouse monoclonal antibody that detects the Innocuous Inntag IT5 based on pollen allergen PhIp2 sequence and is tested for Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected Inntag 5 (IT5) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunoprecipitation Analysis: A representative lot detected Inntag 5 (IT5) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunofluorescence Analysis: A representative lot detected Inntag 5 (IT5) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Biochem/physiol Actions

Clone R19/8-11/18 detects Pollen allergen Phl p 2 in lysate from HEK293 cells transfected with GFP-IT5.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in transfected HEK293/GFP-IT5 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 5 (IT5) in 10 µg of transfected HEK293/GFP-IT5 cell lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable