MABF409

Anti-CD28 Antibody (human), PE, clone CD28.2

clone CD28.2, from mouse

• Synonym(s): T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: PE
• Clone: CD28.2, monoclonal
• Application: FACS, IHC, IP
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CD28.2, monoclonal
• species reactivity: human
• packaging: pkg of 5 μL (0.25 μg/test)
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1κ
• UniProt accession no.: P10747
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD28(940)

Description

General description

The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICCOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo. This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This mouse monoclonal antibody Anti-CD28 (human), PE, clone CD28.2 validated for use in IP, IHC & Flow Cytometry for the detection of CD28.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, <0.2% BSA, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD28 in one million human peripheral blood lymphocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable