MABE283

Anti-p53 Antibody, clone PAb421

clone PAb421, from mouse

• Synonym(s): Cellular tumor antigen p53, Tumor suppressor p53
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: PAb421, monoclonal
• Application: IHC, IP, WB
• Citations: 39

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PAb421, monoclonal
• species reactivity: human, mouse
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001120705
• UniProt accession no.: P02340
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Trp53(22059)

Description

General description

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kDa phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy. Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: Most of them are missense point mutations giving rise to an altered protein function. Also, many, but not all, mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

~53 kDa observed

Immunogen

Epitope: Amino acids 376-378 in human p53

Partially purified mouse p53

Application

Immunoprecipitation Analysis: A representative lot from independent laboratory immunoprecipitated p53 in IP (Lehman, T. A., et al. (1991). Cancer Res. 51(15):4090-4096.; Harlow, E., et al. (1981). J Virol. 39(3):861-869.)

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in human breast cancer tissue (Davidoff, A. M., et al. (1992). Proc Natl Acad Sci USA. 89(8):3439-3442.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-p53 Antibody, clone PAb421 (Mouse Monoclonal Antibody) validated in WB, IP, IHC to detect p53 also known as Cellular tumor antigen p53, Tumor suppressor p53.

Biochem/physiol Actions

This antibody recognizes amino acids 376-378 of human p53.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 in 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable