MABE1006

Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1

clone 17-3-4-1, from mouse

• Synonym(s): N6-methyladenosine, m6A
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 17-3-4-1, monoclonal
• Technique(s): RIP: suitable, dot blot: suitable
• Application: DB, RIP
• Citations: 33

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 17-3-4-1, monoclonal
• species reactivity (predicted by homology): all
• technique(s): RIP: suitable, dot blot: suitable
• isotype: IgG1λ
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Cat. No. MABE1006, Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.

Immunogen

Epitope: N6-methyladenosine

Hapten N6-methyladenosine-5′-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).

Application

(RNA) Immunoprecipitation Analysis: A representative lot immunoprecipitated N6-methyladenosine in IME2 transcripts (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
RNA Dot Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).

Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1 is an antibody against N6-methyladenosine for use in Dot Blot and RNA binding protein immunoprecipitation.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by RNA Dot Blot in oligomers containing N6-methyladenosine.

RNA Dot Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable