MABD51

Anti-Brn-2 (POU3F2) Antibody, clone 8C4.2

clone 8C4.2, from mouse

• Synonym(s): POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8C4.2, monoclonal
• Application: IHC, WB
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8C4.2, monoclonal
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_005595.2
• UniProt accession no.: P20265
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POU3F2(5454)

Description

General description

Brn-2 (POU domain, class 3, transcription factor 2; Oct-7; N-Oct-3; or POU3F2) is a member of a large family of POU-domain transcription factors. The highly homologous POU domain contains a POU-specific domain at the N-terminus, a POU-homeo domain at the C-terminus and an interconnecting linker region. The POU domain binds to the DNA sequence 5’-ATGCAAAT-3’. Brn-2 is highly expressed in the developing CNS and may play a role in neurogenesis, particularly in the development of the hypothalamus. Previous studies have also suggested that Brn-2 integrates signals downstream of the BRAF/MAP kinase and Wnt/β-catenin pathways, and Brn-2 may be involved in the transformation and proliferation of melanomas.

~54 kDa observed.
The calculated molecular weight is 47 kDa Brn-2 (POU3F2) may be observed at ~50-55 kDa in some cell lysates (Wolfe, A., et al., (2002). Molecular Endocrinology. 16(3):435-449).

Immunogen

GST-tagged recombinant protein corresponding human Brn-2 (POU3F2).

Application

Anti-Brn-2 (POU3F2) Antibody, clone 8C4.2 detects level of Brn-2 (POU3F2) & has been published & validated for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Brn-2 in normal rat brain tissue, in Purkinje, basket, and glial cells of rat cerebellum tissue, in neurons of rat cerebellum tissue, in neurons and glial cells of rat frontal cortex tissue, and in neurons of human pons tissue.

Physical form

Format: Purified

Analysis Note

Control
SK-N-MC cell lysates

Evaluated by Western Blot in SK-N-MC cell lysates.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Brn-2 (POU3F2) in 10 µg of SK-N-MC cell lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable