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GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

liquid, clone 528, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor

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Pack SizeSKUAvailabilityPrice
100 μg
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$519.00

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
528, monoclonal
Species reactivity:
human
Application:
—
Citations:
14

$519.00

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

528, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Calbiochem®

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This Item
MABF119GR11SAB4500714
Anti-EGFR (Ab-1) Mouse mAb (528) liquid, clone 528, Calbiochem®

Sigma-Aldrich

GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR Antibody, clone 528 (Azide-free) clone 528, from mouse

Sigma-Aldrich

MABF119

Anti-EGFR Antibody, clone 528 (Azide-free)

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) liquid, clone αIR3, Calbiochem®

Sigma-Aldrich

GR11

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

Anti-EGFR antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4500714

Anti-EGFR antibody produced in rabbit

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, rat, mouse

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)

Gene Information

human ... IGF1R(3480)

Gene Information

human ... EGFR(1956)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

528, monoclonal

clone

528, monoclonal

clone

αIR3, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

form

liquid

form

-

form

liquid

form

buffered aqueous solution

General description

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation(1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Negative Control
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue

Other Notes

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David Cachia et al.
Journal of neuro-oncology, 125(2), 401-410 (2015-09-12)
Gliosarcoma is classified by the World Health Organization as a variant of glioblastoma. These tumors exhibit biphasic histologic and immunophenotypic features, reflecting both glial and mesenchymal differentiation. Gliosarcomas can be further classified into primary (de novo) tumors, and secondary gliosarcomas
F Terzi et al.
The Journal of clinical investigation, 106(2), 225-234 (2000-07-21)
The role of EGF in the evolution of renal lesions after injury is still controversial. To determine whether the EGF expression is beneficial or detrimental, we generated transgenic mice expressing a COOH-terminal-truncated EGF-R under the control of the kidney-specific type
Inmaculada Bañón-Rodríguez et al.
The EMBO journal, 33(2), 129-145 (2014-01-15)
Establishing the correct orientation of the mitotic spindle is an essential step in epithelial cell division in order to ensure that epithelial tubules form correctly during organ development and regeneration. While recent findings have identified some of the molecular mechanisms
Adam C Midgley et al.
The Journal of biological chemistry, 288(21), 14824-14838 (2013-04-17)
Fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine transforming growth factor-β1 (TGF-β1). Myofibroblasts express α-smooth muscle actin and are present in granulation tissue, where they are responsible for wound contraction. Our previous studies
Rozanne Lee et al.
Journal of biomolecular screening, 13(3), 210-217 (2008-03-04)
Monoclonal antibodies (mAb) are not only useful reagents but also represent a promising type of therapeutics due to their high affinity and exquisite specificity for their antigens. A critical step in mAb generation is to identify antigen-specific antibodies. Although enzyme-linked
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Global Trade Item Number

SKUGTIN
GR01-100UG04055977220162

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