GF346

Transforming Growth Factor-β1 human

Animal free, >98%, recombinant, expressed in CHO cells, suitable for cell culture

• Synonym(s): TGFb1, TGFbI, TGFB, Transforming Growth Factor-β1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77
• Impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)

Properties

• Product Name: TGF-β1,; Human Recombinant Animal Free,
• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Segment: 100
• UniProt accession no.: P01137
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Product Source: CHO cells

The three mammalian isoforms of TGF-β (TGF-β1, TGF-β2, TGF-β3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF- isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.

Recombinant human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Physical form

Product is presented without additives and is filtered through a 0.2 micron filter before lyophilization

Preparation Note

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Analysis Note

Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable