The capture antibody in this product is a polyclonal guinea pig anti-human insulin antibody. The detection antibody is a monoclonal antibody that recognizes the region specific for intact human proinsulin. Epitope mapping has not been performed.
EZHPI
Human Proinsulin ELISA Kit
measures and quantifies Proinsulin levels in 20 μL serum or plasma
Synonym(s):
Human Proinsulin Detection, Proinsulin ELISA
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 96 wells | Check Cart for Availability | $815.00 |
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Input:
sample type serum
sample type plasma (K2 EDTA)
sample type plasma (K2 EDTA)
Assay range:
accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice
$815.00
Check Cart for Availability
Product Name
Human Total Proinsulin ELISA, This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.
Quality Level
species reactivity
human
packaging
kit of 1 × 96 wells
parameter
20 μL sample volume (4hr assay)
assay range
accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM
technique(s)
ELISA: suitable
input
sample type serum
sample type plasma (K2 EDTA)
NCBI accession no.
UniProt accession no.
application(s)
research use
detection method
colorimetric (450nm)
shipped in
wet ice
storage temp.
2-8°C
General description
Proinsulin, the precursor of insulin and C-peptide, is made by the β cells of the pancreas. Proinsulin formed by the cleavage of pre-proinsulin contains B- and A-domains linked through the connecting domain. Proinsulin interacts with insulin receptor-A (IR-A) isoform present in the nervous system and exhibits antiapoptotic and neuroprotective effects in the developing and postnatal nervous system. Elevated levels of proinsulin serve as a marker for secretory β-cell dysfunction. Increased levels of this hormone are also observed in patients with type 2 diabetes and impaired glucose tolerance. This kit measures the intact and intermediate metabolic forms of human proinsulin.
Application
Human Total Proinsulin ELISA has been used to measure plasma, serum, eye, and retinal levels of human proinsulin.
Other Notes
Research Sub Category
Obesity
Metabolic Disorders
Research Category
Metabolism
20 μL sample volume 3 hour assay, room temperature
Obesity
Metabolic Disorders
Research Category
Metabolism
20 μL sample volume 3 hour assay, room temperature
Disclaimer
For research use only. Not for use in diagnostic procedures.
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This Item | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| assay range accuracy: 88-103%, sensitivity: 0.5 pM, linearity: 84-120%, standard curve range: 2-200 pM | assay range accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL | assay range accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL | assay range accuracy: 94-114%, intra-assay cv: 1.4-4.9 |
| input sample type serum | input sample type plasma (K2 EDTA) | input sample type cell culture supernatant | input sample type cell culture supernatant |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
| detection method colorimetric (450nm) | detection method colorimetric (450nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) |
signalword
Warning
hcodes
Hazard Classifications
Met. Corr. 1 - Skin Sens. 1
wgk
WGK 3
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Carolina Isiegas et al.
Investigative ophthalmology & visual science, 57(8), 3610-3618 (2016-07-09)
The induction of proinsulin expression by transgenesis or intramuscular gene therapy has been shown previously to retard retinal degeneration in mouse and rat models of retinitis pigmentosa (RP), a group of inherited conditions that result in visual impairment. We investigated
Flora de Pablo et al.
Frontiers in molecular neuroscience, 11, 426-426 (2018-12-12)
Proinsulin was first identified as the primary translation product of the insulin gene in Donald Steiner's laboratory in 1967, and was the first prohormone to be isolated and sequenced. While its role as an insulin precursor has been extensively studied
Michael A Weiss
The Journal of biological chemistry, 284(29), 19159-19163 (2009-04-28)
Insulin plays a central role in the regulation of vertebrate metabolism. The hormone, the post-translational product of a single-chain precursor, is a globular protein containing two chains, A (21 residues) and B (30 residues). Recent advances in human genetics have
Global Trade Item Number
| SKU | GTIN |
|---|---|
| EZHPI-15K | 04053252320965 |
| EZHPI-15BK | 04053252436994 |
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What type of antibody is used in the EZHPI-15K kit to detect human pro-insulin? Is it known what epitopes it is binding and if they are conformational or linear? Is it polyclonal or monoclonal?
1 answer-
Helpful?
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