CBL194F

Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated

clone LP5K, Chemicon^®, from mouse

• Synonym(s): Cytokeratin 7 Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: LP5K, monoclonal
• Application: FACS, ICC, IHC (p)
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: saturated ammonium sulfate (SAS) precipitated
• antibody product type: primary antibodies
• clone: LP5K, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG2b
• NCBI accession no.: NM_005556.3
• UniProt accession no.: P08729
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... KRT7(3855)

Description

Immunogen

Cytoskeletal proteins from cultured HeLa cells

Application

Detect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC.

Immunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).

Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).

Flow cytometry: 10μL of conjugate per test.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Biochem/physiol Actions

The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

FUSION PARTNER: Sp2/0

Physical form

The antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.

Preparation Note

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Analysis Note

Tested

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2