As part of the protocol for CBA415, one must induce cytotoxicity. Therefore, we have not studied maintaining live organoids after using this kit. Please refer to the protocol for explanations of this kit's functions.
CBA415
Live Dead Cell Viability Assay Kit for 3D and 2D cell culture
Live-dead cell viability kit for 3D and 2D cell cultures. Indicates live, dead and total cell count. Sufficient for 5 x 24-well plates or 12 x 96-well plates.
Synonym(s):
Viability Assay Kit
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
Check Cart for Availability | $685.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
12352207
$685.00
Check Cart for Availability
detection method
fluorometric
1 of 1
This Item | |||
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| detection method fluorometric | detection method fluorometric | detection method - | detection method chemiluminescent |
General description
The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells), Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids, human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry, fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity.
One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.
<bold>Advantages:</bold>
1. Tested and optimized for 3D cell culture, Spheroid culture, organoid culture, 2D cell culture and flow cytometry.
2. Easy to use and quick protocol.
3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.
Spectral Properties
Calcein AM: Ex/Em: 490/515 nm (Green)
Propidium Iodide: Ex/Em: 535/617 nm (Red)
Hoechst 33342: Ex/Em: 361/486 nm (Blue)
Protocols:
Protocols for staining 2D monolayer cell culture, 3D cell culture, 3D spheroids and 3D organoids are included in the datasheet.
One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.
<bold>Advantages:</bold>
1. Tested and optimized for 3D cell culture, Spheroid culture, organoid culture, 2D cell culture and flow cytometry.
2. Easy to use and quick protocol.
3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.
Spectral Properties
Calcein AM: Ex/Em: 490/515 nm (Green)
Propidium Iodide: Ex/Em: 535/617 nm (Red)
Hoechst 33342: Ex/Em: 361/486 nm (Blue)
Protocols:
Protocols for staining 2D monolayer cell culture, 3D cell culture, 3D spheroids and 3D organoids are included in the datasheet.
Preparation Note
Store at -20oC or below, and protect from light. The vials can be frozen and thawed upto 10 times. Before use, allow the vials to thaw to room temperature and centrifuge briefly.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| CBA415 | 04061842097074 |
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Is it possible mantain alive organoids after using this kit?
1 answer-
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Could I know the exact concentration of each components(Calcein-AM, PI, Hoechst) ? I already understood your recommendations on the puroduct information sheet, but we need to know the exact concentration with units. ex.) uM or ug/ml
1 answer-
The exact concentrations of Calcein-AM, PI, and Hoechst are proprietary and cannot be disclosed.
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Is it possible to use formaldehyde or formalin for fixing cells before utilizing this live/dead cell staining solution?
1 answer-
The use of this kit on formaldehyde or formalin fixed cells has not been assessed in our lab, and our literature survey has not provided a recommended protocol reference.
It's important to note that fixing cells with a crosslinker such as formalin or formaldehyde could potentially impact the results of this assay. The harsh conditions of crosslinking may lead to cell membrane damage and increased cell death. As this has not been tested in our lab, we highly recommend conducting a pilot experiment to confirm compatibility.
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Could this product be used for live imaging for 72hours ?
1 answer-
Working solutions of the Propidium Iodide and the Hoechst 33342 are stable for at least 72 hours when refrigerated and protected from light. Calcein AM, once diluted to a working concentration in a aqueous diluent, will begin to degrade within several hours and storage is not recommended.
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How long can I keep the dye solution for after I add the dyes in the PBS and media?
1 answer-
The dye solution for cell staining should be prepared just before use. Store at -20oC or below, and protect from light. The vials can be frozen and thawed up to 10 times. Before use, allow the vials to thaw to room temperature and centrifuge briefly.
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