AP136P

Goat Anti-Rat IgG Antibody, HRP conjugate

Chemicon^®, from goat

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA, IHC, WB
• Citations: 75

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Goat anti-rat IgG affinity purified antibody, HRP conjugated.

Application

Detect Rat IgG using this Goat anti-Rat IgG Antibody, HRP conjugate validated for use in ELISA, IH & WB.

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Specific for rat IgG, heavy and light chain.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitute with 2 mL of sterile distilled water.

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Analysis Note

Routinely evaluated on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable