AM80

Anti-PAR (Ab-1) Mouse mAb (10H)

liquid, ≥95% (SDS-PAGE), clone 10H

• Synonym(s): Anti-Poly (ADP-Ribose)
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 10H, monoclonal
• Species reactivity: human, Drosophila, rat, mouse
• Application: —
• Citations: 31

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 10H, monoclonal
• assay: ≥95% (SDS-PAGE)
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, Drosophila, rat, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (2.5 µg/mL); Immunocytochemistry (5-20 µg/mL))
• isotype: IgG3
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.

Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine

purified poly(ADP-ribose)

Application

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Normal mouse IgG

Positive Control
Etoposide-treated HL-60 cells

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.

PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable