ABS45

Anti-phospho-MYPT1 (Thr696) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: WB, inhibition assay
• Citations: 46

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: (~130 kDa observed; 115.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): canine (based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), horse (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• technique(s): inhibition assay: suitable (Peptide Inhibition Assay), western blot: suitable
• NCBI accession no.: NP_001137357.1
• UniProt accession no.: O14974
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr696)
• Gene Information: human ... PPP1R12A(4659)

Description

General description

Anti-phospho-MYPT1 (Thr696), Cat. No. ABS45, is a rabbit polyclonal antibody that detects MYPT1 phosphorylated on Threonine 696 and is tested for use in Western Blotting and Peptide Inhibition Assay.

Protein phosphatase 1 regulatory subunit 12A (UniProt: O14974; also known as Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 4659) in human. Myosin light chain phosphatase with its regulatory subunit, myosin phosphatase target subunit 1 (MYPT1), modulates Ca2+-dependent phosphorylation of myosin light chain by myosin light chain kinase, which is essential for smooth muscle contraction. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, which inhibits myosin phosphatase through the action of Rho-kinase. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. It is phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696 that inhibits PP1C activity by an intramolecular mechanism. Mutations in PPP1R12A are involved in Genitourinary and/or brain malformation syndrome (GUBS), an autosomal dominant syndrome that is characterized by multiple congenital anomalies including urogenital malformations and brain abnormalities. Five isoforms of MYPT1 have been described that are produced by alternative splicing. (Ref.: Qiao, Y-N., et al. (2014). J. Biol. Chem. 289(32); 22512-22523).

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Immunogen

Epitope: Phosphorylated Thr696

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphothreonine 696 of human MYPT1.

Application

Anti-phospho-MYPT1 (Thr696) Antibody is an antibody against phospho-MYPT1 (Thr696) for use in WB.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blotting (SNAP ID)Analysis: 0.1 µg/mL from a previous lot detected MYPT1 on 10 µg of NIH/3T3 cell lysate.

Biochem/physiol Actions

This rabbit polyclonal antibody specifically detects MYPT1 phosphorylated on threonine 696.

Physical form

Affinity purified

Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Calyculin/Okadaic treated and untreated HEK293 cell lysates

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 07-251

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable