ABE483

Anti-TFAM Antibody

serum, from rabbit

• Synonym(s): Transcription factor A, mitochondrial, mtTFA, Testis-specific high mobility group protein, TS-HMG
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 14

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_033386
• UniProt accession no.: P40630
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TFAM(7019) ; mouse ... Tfam(21780)

Description

General description

TFAM, also called mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development.

~27 kDa observed

Immunogen

His-tagged recombinant protein corresponding to mouse mtTFA.

Application

Detect TFAM using this rabbit polyclonal antibody, Anti-TFAM Antibody validated for use in western blotting & IP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TFAM in C2C12 cell lysate (Prof. B Kaufman, University of Pennsylvania.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFAM in 10 µg of C2C12 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1