ABE25

Anti-acetyl-c-Myc (Lys148) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), sheep (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_002458
• UniProt accession no.: P01106
• shipped in: wet ice
• target post-translational modification: acetylation (Lys148)
• Gene Information: bovine ... Myc(511077) ; chimpanzee ... Myc(464393) ; dog ... Myc(403924) ; human ... MYC(4609) ; mouse ... Myc(17869) ; pig ... Myc(448810) ; rat ... Myc(24577) ; rhesus monkey ... Myc(694626) ; sheep ... Myc(443447)

Description

General description

c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.

Specificity

This antibody recognizes c-Myc acetylated at Lys148 in human and Lys149 in mouse.

Immunogen

Epitope: Acetylated Lys148

KLH-conjugated linear peptide corresponding to human c-Myc acetylated at Lys148.

Application

Peptide Inhibition Assay Analysis: 2 µg/mL from a representative lot blocked acetyl-c-Myc (Lys148) in HEK293T cells co-transfected with wildtype c-Myc and P300.

Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-acetyl-c-Myc (Lys148) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-c-Myc (Lys148) also known as HLHe39, Proto-oncogene c-Myc, Transcription factor p64.

Quality

Evaluated by Western Blot in transfected HEK293 cell lysates.

Western Blot Analysis: 0.1 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293 cell lysates.

Target description

~58 kDa observed. Uniprot describes two isoforms at 49 and 51 kDa Uncharacterized bands appear at ~13 and ~17 in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Transfected HEK293 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable