ABC217
Anti-GLI1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GLI1(2735)
General description
Zinc finger protein GLI1 is also called Glioma-associated oncogene and Oncogene GLI. GLI1 is a transcriptional activator that regulates specific genes during normal development. GLI1 also mediates SHH signaling (and therefore cell proliferation and differentiation) and possibly is involved in craniofacial, central nervous system, gastrointestinal tract, and digital development. GLI1 is expressed in the testis, myometrium, fallopian tube, and brain.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human GLI1.
Application
Anti-GLI1 Antibody is a highly specific rabbit polyclonal antibody, that targets Gli1 & has been tested in western blotting & IHC (Paraffin).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting Analysis: A 1:500 dilution from a representative lot detected GL1 in A431, HEK293, HEPG2, and PC3 cells.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GLI1 in human cerebellum and thalamus tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GLI1 in rat testis tissue.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GLI1 in human cerebellum and thalamus tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GLI1 in rat testis tissue.
Quality
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected GLI1 in 10 µg of A549 cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected GLI1 in 10 µg of A549 cell lysate.
Target description
~150 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Linkage
Replaces: MABC678
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Natalia Garcia et al.
Reproductive sciences (Thousand Oaks, Calif.), 29(3), 781-790 (2021-10-14)
Uterine leiomyosarcoma (LMS) contributes to a significant proportion of uterine cancer deaths. It is a rare and high-risk gynecological cancer. LMS is challenging to the treatment due to the resistance of several therapies. The activation of the Hedgehog (HH) pathway
Effects of long-term exposure to MST-312 on lung cancer cells tumorigenesis: Role of SHH/GLI-1 axis.
Nicolas Jones Villarinho et al.
Cell biology international, 46(9), 1468-1479 (2022-07-12)
Replicative immortality is a key feature of cancer cells and it is maintained by the expression of telomerase, a promising target of novel therapies. Long-term telomerase inhibition can induce resistance, but the mechanisms underlying this process remain unclear. The Sonic
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