ABC142
Anti-Pex14 (peroxisomal membrane marker) Antibody
serum, from rabbit
Synonym(s):
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μL | Check Cart for Availability | $534.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
8
$534.00
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A recombinant, preservative-free antibody is available for your target. Try ZRB2470
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
technique(s)
immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PEX14(5195)
General description
Peroxisomal membrane protein 14 (PEX14) is a component of the peroxisomal translocation machinery. It interacts with PEX19 and PEX13, and functions as the peroxisomal membrane docking factor for PEX5. The PEX7-PTS2 complex also docks at PEX14 indirectly via PEX5L, the longer isoform of PEX5. Defects in PEX14 result in peroxisome biogenesis disorder complementation group K and Zellweger syndrome.
~60 kDa observed. This product is known to exist in two isoforms at 41 kDa and 37 kDa, but it has also been observed at ~57-60 kDa (Nguyen, T. et al. 2006. J Cell Sci. 119 (Pt 4):636-645). An uncharacterized band may be observed at ~51 kDa in some cell lysates.
Immunogen
His-tagged recombinant protein corresponding to human PEX14.
Application
Detect Pex14 (peroxisomal membrane marker) using this Anti-Pex14 (peroxisomal membrane marker) Antibody validated for use in Western Blotting, ICC.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PEX14 (peroxisomal membrane marker) in 10 µg of human liver tissue lysate.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PEX14 (peroxisomal membrane marker) in Pex14p-transfected CHO cells (Pinto, M. P., et al. 2006. J Biol Chem. 281(45):34492-34502.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PEX14 (peroxisomal membrane marker) in Pex14p-transfected CHO cells (Pinto, M. P., et al. 2006. J Biol Chem. 281(45):34492-34502.).
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution from a representative lot detected PEX14 (peroxisomal membrane marker) in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution from a representative lot detected PEX14 (peroxisomal membrane marker) in 10 µg of NIH/3T3 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| antibody form serum | antibody form affinity isolated antibody | antibody form serum | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
| technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000 | technique(s) western blot: suitable | technique(s) indirect ELISA: suitable |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lorena Benedetti
Current protocols, 1(3), e71-e71 (2021-03-04)
Intracellular signaling processes are frequently based on direct interactions between proteins and organelles. A fundamental strategy to elucidate the physiological significance of such interactions is to utilize optical dimerization tools. These tools are based on the use of small proteins
Scott E Fuller et al.
Journal of applied physiology (Bethesda, Md. : 1985), 127(1), 143-156 (2019-05-17)
Adaptations in hepatic and skeletal muscle substrate metabolism following acute and chronic (6 wk; 5 days/wk; 1 h/day) low-intensity treadmill exercise were tested in healthy male C57BL/6J mice. Low-intensity exercise maximizes lipid utilization; therefore, we hypothesized pathways involved in lipid
Li-Chun Cheng et al.
Nucleus (Austin, Tex.), 10(1), 126-143 (2019-05-31)
The double membrane nuclear envelope (NE), which is contiguous with the ER, contains nuclear pore complexes (NPCs) - the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) - a scaffold for NE and chromatin organization. Since numerous human diseases linked to NE
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABC142 | 04053252599644 |