AB9082

Anti-Ryanodine Receptor 3 Antibody

serum, Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC, WB
• Citations: 16

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_001036.3
• UniProt accession no.: Q15413
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... RYR3(6263)

Description

Immunogen

Synthetic peptide from the variant TM region of human Ryanodine Receptor 3.

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience

This Anti-Ryanodine Receptor 3 Antibody is validated for use in IC, IH, WB for the detection of Ryanodine Receptor 3.

Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 3 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Ryanodine Receptor 3.

Physical form

Rabbit serum. Liquid.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Western blot = adult mouse brain (adult mouse skeletal muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable