AB761

Anti-Collagen Type II (COL2A1) Antibody

CHEMICON^®, rabbit polyclonal

• Synonym(s): Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: ELISA, ICC, IHC, WB
• Citations: 10

Properties

• Product Name: Anti-Collagen Type II Antibody, Chemicon^®, from rabbit
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_001844.4, NM_033150.2
• UniProt accession no.: P02458
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... COL2A1(1280)

Description

Immunogen

Collagen type II extracted and purified from human cartilage.

Application

Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.

ELISA 1:1000.

Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20

Western Blot 1:100

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Biochem/physiol Actions

The antibody reacts with both native and denatured human collagen type II.



Cross Reactivity Percent

Human collagen type II 90%

Human collagen type I, III, IV, V <1%

Human plasma proteins do not interfere with binding to collagen.

Physical form

Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.

Product is lyophilized.

Preparation Note

Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360FD
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects