AB1585

Anti-Dopamine β Hydroxylase Antibody

serum, Chemicon^®

• Synonym(s): DBH, Dopamine beta-monooxygenase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, RIA, WB
• Citations: 36

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: bovine, guinea pig, rat, human, feline, rabbit
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable
• NCBI accession no.: NM_000787.3
• UniProt accession no.: P09172
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DBH(1621)

Description

General description

69 kDa

Immunogen

Native enzyme from bovine adrenal glands.

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody validated for use in IH, FUNC, RIA & WB.

Immunohistochemistry: 1:2,000 on rabbit and guinea pig using an ABC detection method.

Western blot

RIA

Inhibitory studies in vivo and in vitro.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes Dopamine beta hydroxylase. AB1585 reacts with a single band on Western blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme.

Physical form

Rabbit Serum. Lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Unpurified

Preparation Note

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol can be added 1:1 for additional stability.

Analysis Note

Control
Brain (Striatum/Substantia Nigra)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB145

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable