AB144

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, goat polyclonal

• Synonym(s): ChAT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: guinea pig, mouse, rat
• Application: IHC, WB
• Citations: 120

Properties

• Product Name: Anti-Choline Acetyltransferase Antibody, serum, Chemicon^®
• biological source: goat
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: guinea pig, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_020549.3, NM_020984.2, NM_020985.2, NM_020986.2
• UniProt accession no.: P28329
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Chat(12647) ; rat ... Chat(290567)

Description

General description

70 kDa

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Immunogen

Human placental enzyme.

Application

Anti-Choline Acetyltransferase Antibody is an antibody against Choline Acetyltransferase for use in IH & WB.

Immunoblot: 1:1,000-1:5,000

Immunohistochemistry: 1:500-1:1,000. See page two of this data sheet.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes cholinergic neurons in brain and central nervous systems.

Physical form

Goat antisera, liquid, no preservatives.

Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable