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71259

Insect GeneJuice® Transfection Reagent

Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.

Synonym(s):

GeneJuice Transfection

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0.3 mL
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$149.00
1 mL
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$295.00
10 mL
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$2,120.00

About This Item

NACRES:
NA.54
UNSPSC Code:
41106502
Form:
suspension
Technique(s):
transfection: suitable

$149.00


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Quality Segment

form

suspension

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

transfection: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Insect GeneJuice<TMSYMBOL></TMSYMBOL> Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx<TMSYMBOL></TMSYMBOL> and pBiEx<TMSYMBOL></TMSYMBOL> vectors, which contain the immediate early baculovirus promoter, IE1.

  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 75,726 when other reagent used with Sf9, thus 1.7 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 11,952 when other reagent used with High Five, thus 11 fold difference.
  • When Insect Genejuice® used in conjunction with Sf9 cell line, raw relative light units (RLUs) from Renilla Luciferase expression with plex is 131,90 Vs. 31,897 when Insect Genejuice used with High Five, thus 4.1 fold difference.

Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.

Legal Information

GeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
709677128171115
form

suspension

form

liquid

form

liquid

form

liquid

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

do not freeze

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice


Storage Class

12 - Non Combustible Liquids



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With our protein expression platforms, you can choose the proteins you purify to answer the precise biological questions you’re tackling. Don’t let limitations like solubility, yield, or toxicity influence your choice of proteins to study. With the expertise of Novagen®, the household name in cloning and recombinant protein expression technology, we make it possible to elucidate meaningful functions of even the most intractable gene products.

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Global Trade Item Number

SKUGTIN
71259-EA04055977333077
71259-307790788053123
71259-50UL04053252045073
71259-404055977270860
71259-504055977270877

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