5.09583

Diaphanous (mDia)-related Formin Agonist, IMM01

• Synonym(s): Diaphanous (mDia)-related Formin Agonist, IMM01, Formin Activator
• Empirical Formula (Hill Notation): C₁₂H₁₇N₃O₂S
• CAS Number: 218795-74-5
• Molecular Weight: 267.35
• MDL number: MFCD00021639
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder
• Storage condition: OK to freeze, protect from light

Properties

• assay: ≥98% (HPLC)
• Quality Segment: 100
• form: powder
• potency: 140 nM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: off-white
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC₅₀ = 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 µM) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Primary Target
mDia

Reversible: yes

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lash, L.L., et al. 2013. Cancer Res.73, 6793.
Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable