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480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Synonym(s):

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase

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500 mI.U.
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$516.00

About This Item

CAS Number:
9001-67-6
EC Number:
3.2.1.18 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
MFCD00149915
Specific activity:
≥10 units/mL, ≥250 units/mg protein
Recombinant:
expressed in E. coli

$516.00

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recombinant

expressed in E. coli

Quality Segment

100

description

RTECS - QQ3450000

product line

EMPROVE® EXPERT

form

liquid

specific activity

≥10 units/mL, ≥250 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
Note: 1 mU = 1 milliunit.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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This Item
N2133N2876N3001
α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Sigma-Aldrich

480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Neuraminidase from Clostridium perfringens (C. welchii) Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

Sigma-Aldrich

N2133

Neuraminidase from Clostridium perfringens (C. welchii)

Neuraminidase from Clostridium perfringens (C. welchii) Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder

Sigma-Aldrich

N2876

Neuraminidase from Clostridium perfringens (C. welchii)

Neuraminidase from Clostridium perfringens (C. welchii) Type VI, lyophilized powder, ≥6 units/mg protein (using 4MU-NANA), ≥2 units/mg protein (mucin)

Sigma-Aldrich

N3001

Neuraminidase from Clostridium perfringens (C. welchii)

specific activity

≥10 units/mL, ≥250 units/mg protein

specific activity

≥50 units/mg protein (using 4MU-NANA)

specific activity

≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA)

specific activity

2-10 units/mg protein (mucin), 6-15 units/mg protein (using 4MU-NANA)

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

liquid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

-

storage condition

do not freeze

storage condition

-

storage condition

-

storage condition

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

12 - Non Combustible Liquids

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Global Trade Item Number

SKUGTIN
480708-500MIU04055977201420

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