426205

β-Lactamase, Bacillus cereus 569/H9

• Synonym(s): β-Lactamase, Bacillus cereus 569/H9, Penicillinase
• CAS Number: 9073-60-3
• EC Number: 3.5.2.6 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00131819
• Biological source: Bacillus sp. (Bacillus cereus)

Properties

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Segment: 100
• description: Merck USA index - 14, 7093
• form: lyophilized
• packaging: vial of ≥50 units β-lactamase II, vial of ≥500 units β-lactamase I
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: sterile distilled water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

β-Lactamase produced by bacteria shares sequence homology to penicillin-binding proteins.[1] This enzyme is found in Gram-negative bacteria.[2] β-Lactamases have four molecular classes, named A, B, C, and D. A, C, and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes, which have a zinc ion at their active site for β-lactam hydrolysis.[3]

Biochem/physiol Actions

β-Lactamase hydrolyzes β-lactum antibiotics and is the chief cause of resistance to β-lactam antibiotics development by bacteria.[1][3] Mutations in the β-lactamases are associated with extended-spectrum β-lactamases (ESBLs).[4]

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 month at 4°C.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol benzyl penicillin and 1.0 µmol of cephalosporin C, respectively, per min at 25°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable