The activity is assessed in "IU" using an inhibition assay, with the result corresponding to a specific activity in international units per milligram (IU/mg).
407320
γ-Interferon, Mouse, Recombinant, E. coli
Synonym(s):
Interferon γ precursor, IFN-γ, rmIFN-γ
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 μg | Check Cart for Availability | $289.00 | |
| 100 μg | Check Cart for Availability | $710.00 |
About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥95% (RP-HPLC, FPLC, and SDS-PAGE)
assay
≥95% (RP-HPLC, FPLC, and SDS-PAGE)
Quality Segment
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
solubility
soluble (Reconstitute with sterile water to >=100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.)
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Biochem/physiol Actions
Reported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.
Physical form
Lyophilized from 0.5X PBS, pH 7.2.
Preparation Note
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
Other Notes
Alvarado, J., et al. 2005. Immunol. Lett.99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| assay ≥95% (RP-HPLC, FPLC, and SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay >98% (SDS-PAGE and HPLC) | assay - |
| form lyophilized | form liquid | form liquid | form liquid |
| solubility soluble (Reconstitute with sterile water to >=100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.) | solubility water: soluble | solubility - | solubility - |
| storage temp. −20°C | storage temp. −70°C | storage temp. -10 to -25°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
| storage condition OK to freeze, desiccated | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition - | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 407320-100UG | 04055977188318 |
| 407320-20UG | 04055977188325 |
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How can product 407320-20UG be measured in IU for a dose concentration of 100 IU/mL for an experiment, considering that the label specifies a specific activity of 10 million U/mg P? Is there any interchangeability between these units and the ones I require?
1 answer-
Helpful?
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