405210

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

• Synonym(s): ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
• Empirical Formula (Hill Notation): C₁₆H₁₄N₂O₄
• CAS Number: 147591-46-6
• Molecular Weight: 298.29
• MDL number: MFCD00593367
• UNSPSC Code: 12352200
• NACRES: NA.51
• Assay: ≥95% (HPLC)
• Form: powder
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [N+](=O)([O-])c1c2c([n](c1C)c3ccc(cc3)OC)cc(cc2)O
• InChI: 1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3
• InChI key: VVZNWYXIOADGSW-UHFFFAOYSA-N

Description

General description

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Packaging

Packaged under inert gas

Other Notes

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3