36-003

Anti-phospho-MYPT1 (Thr850) Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-GUBS, Anti-M130, Anti-MBS, Anti-MYPT1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: chicken, rat, human, mouse
• Application: WB
• Citations: 43

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: chicken, rat, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_002480.1
• UniProt accession no.: O14974
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr850)
• Gene Information: human ... PPP1R12A(4659)

Description

General description

80 kDa

Immunogen

Peptide corresponding to amino acid residues 845-855 of chicken MYPT1; this sequence is identical to amino acids 848-858 of human MYPT1, with the phosphorylated Thr at position 853.

Application

Detect phospho-MYPT1 (Thr850) using this Anti-phospho-MYPT1 (Thr850) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Biochem/physiol Actions

Recombinant truncated chicken MYPT1 phosphorylated on Thr850.

Physical form

Format: Purified

Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.

Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
MYPT1 substrate phosphorylated by ROKa/ROCK-II

Routinely evaluated by immunoblot on phophorylated MYPT1 substrate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1