260960

DNA-PK Inhibitor

The DNA-PK Inhibitor, also referenced under CAS 20357-25-9, controls the biological activity of DNA-PK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): DNA-PK Inhibitor, DMNB, 4,5- Di methoxy-2- nitro benzaldehyde, DNA-Dependent Protein Kinase Inhibitor, DMNB, 4,5-Dimethoxy-2-nitrobenzaldehyde, DNA-Dependent Protein Kinase Inhibitor
• Empirical Formula (Hill Notation): C₉H₉NO₅
• CAS Number: 20357-25-9
• Molecular Weight: 211.17
• UNSPSC Code: 12352100
• NACRES: NA.77
• MDL number: MFCD00007134
• Assay: ≥90% (GC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥90% (GC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: bright yellow
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [N+](=O)([O-])c1c(cc(c(c1)OC)OC)C=O
• InChI: 1S/C9H9NO5/c1-14-8-3-6(5-11)7(10(12)13)4-9(8)15-2/h3-5H,1-2H3
• InChI key: YWSPWKXREVSQCA-UHFFFAOYSA-N

Description

General description

A cell-permeable vanillin derivative that acts as a potent and selective inhibitor of DNA-PK (DNA-dependent protein kinase) activity (IC₅₀ = 15 µM) and DNA-PK-mediated DSB (double strand break) DNA repair by NHEJ (non-homologous DNA-end-joining). Shown to effectively sensitize cells to killing by Cisplatin (Cat. No. 232120 ). Does not affect PKC or Chk2 kinase activities.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
DNA-PK activity

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 15 µM against DNA-PK (DNA-dependent protein kinase) activity

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Willmore, E., et al. 2004. Blood,103, 4659.
Durant, S., and Karran, P. 2003. Nucleic Acids Res.31, 5501.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Carcinogenic / Teratogenic (D)

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable