217570

Anti-CD3 Mouse mAb (UCHT1)

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

• Synonym(s): Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: UCHT1, monoclonal
• Species reactivity: human
• Application: —
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: UCHT1, monoclonal
• assay: >95% (immunoglobulin, SDS-PAGE)
• form: liquid
• contains: 0.5 mg/mL gentamycin sulfate as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections ; Immunoprecipitation ; T cell Co-stimulation )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.

Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

Immunogen

human thymocytes/Sezary T cells

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Other Notes

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.

This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵ cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable