The presence of FBS in the cell culture supernatant containing IgG2b may lead to reduced yield and purity of the mAb, IgG2b, as FBS contains bovine immunoglobulins that can compete with the target antibody for binding to the protein G column.
16-266
Protein G Agarose, Fast Flow
Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.
Synonym(s):
Protein G resin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mL | Check Cart for Availability | $982.00 |
About This Item
UNSPSC Code:
41116133
NACRES:
NA.56
eCl@ss:
32160801
Form:
liquid
$982.00
Check Cart for Availability
form
liquid
manufacturer/tradename
Upstate®
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable
shipped in
wet ice
General description
Fast flow agarose comprises highly crosslinked agarose beads or gels that can tolerate high pressures compared to standard agarose bead resins. Thus, these resins are ideal for fast protein liquid chromatography (FPLC) purification methods. They are preferred for medium to large-scale antibody purification. This product contains protein G covalently coupled by cyanogen bromide to highly cross-linked 4% agarose beads. It is stable in all aqueous buffers used in protein G chromatography.
Application
Physical form
water containing 20% ethanol
Preparation Note
1 year at 4°C
Analysis Note
routinely evaluated by immunoprecipitation and purification
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) ChIP: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form suspension |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Roche |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
116.6 °F
flash_point_c
47 °C
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 16-266 | 04053252325731 |
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Is the 16-266 protein G column compatible with purifying IgG in the presence of FBS when purifying mAb, IgG2b, from cell culture supernatant containing FBS?
1 answer-
Helpful?
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What is the approximate diameter of each agarose bead?
1 answer-
The diameter range of 4% beaded agarose typically falls between 50 to 165 micrometers.
Helpful?
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