The mass load is very much dependent on the specific method and analytes. However, for this size column, the mass load would typically range from 1 - 3 mg.
1.50460
SeQuant® ZIC®-pHILIC (5 µm) HPLC Columns
L x I.D. 150 mm x 2.1 mm
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 unit | Check Cart for Availability | $1,720.00 |
About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC
Pore size:
100 Å pore size
Matrix:
polymeric particle platform
$1,720.00
Check Cart for Availability
Product Name
SeQuant® ZIC®-pHILIC 5µm polymer 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Segment
agency
suitable for USP L122
product line
SeQuant®
feature
endcapped: no
parameter
200 bar pressure, 50 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
15 cm × 2.1 mm
matrix
polymeric particle platform
matrix active group
ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC
particle size
5 μm
pore size
100 Å pore size
operating pH
2-10
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.
The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
SeQuant® ZIC®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds.
Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant® ZIC®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD).
SeQuant® ZIC®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant® ZIC®-HILIC columns with identical functional groups, and SeQuant® ZIC®-cHILIC columns with complementary selectivity.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
SeQuant® ZIC®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds.
Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant® ZIC®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD).
SeQuant® ZIC®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant® ZIC®-HILIC columns with identical functional groups, and SeQuant® ZIC®-cHILIC columns with complementary selectivity.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Theoretical Plates (N/m) (Cytosine): ≥ 40000
Symmetry (Cytosine): 0.95 - 1.40
Symmetry (Cytosine): 0.95 - 1.40
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| matrix active group Zwitterion bonding phase, ZIC-pHILIC | matrix active group Zwitterion bonding phase, ZIC-pHILIC | matrix active group Zwitterion bonding phase, ZIC-pHILIC | matrix active group Zwitterion bonding phase, ZIC-pHILIC |
| separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) |
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 5 μm |
| technique(s) HPLC: suitable, UHPLC-MS: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High) | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High) | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High) |
| agency suitable for USP L122 | agency suitable for USP L122 | agency suitable for USP L122 | agency suitable for USP L122 |
| pore size 100 Å pore size | pore size 100 Å pore size | pore size 100 Å pore size | pore size 100 Å pore size |
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1504600001 | 04027269134426 |
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Hi, I am wondering what the maximum mass load of analyte is for the SeQuant ZIC-pHILIC 150x2.1 mm column. Thanks
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